Increased Circulating Th17 Cells, Serum IL-17A, and IL-23 in Takayasu Arteritis.

Durga Prasanna Misra,Smriti Chaurasia,Ramnath Misra

doi:10.1155/2016/7841718

Durga Prasanna Misra, Smriti Chaurasia + Show 1 more

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https://doi.org/10.1155/2016/7841718

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Abstract

Introduction. Th17, γδT, NK, and NKT cells in peripheral blood and serum IL-17 and IL-23 in Takayasu arteritis (TA) were measured and correlated with disease activity. Methods. Th17 (anti-CD3APC, CD4PECy7, and IL-17PE), NKT, NK (anti-CD3APC, CD56FITC), and γδT (anti-CD3FITC and γδTCRAPC) cells were enumerated by flow cytometry in peripheral blood of 30 patients with TA (ACR1990 criteria) and 20 healthy controls, serum IL-17 and IL-23 measured by ELISA. Relation with disease activity (NIH criteria, ITAS2010) was analyzed (using nonparametric tests, median with interquartile range). Results. Mean age of patients was 33.47 ± 11.78 years (25 females); mean symptom duration was 7.1 ± 5.3 years. 13 were not on immunosuppressants; 12 were active (ITAS2010 ≥ 4). The percentage of Th17 cells was significantly expanded in TA (patients 2.1 (1.5–3.2) versus controls 0.75 (0.32–1.2); p < 0.0001) with no differences in other cell populations. Serum IL-17 and IL-23 (pg/mL) in patients (6.2 (4.6–8.5) and 15 (14.9–26.5), resp.) were significantly higher (p < 0.001) than controls (3.9 (3.9–7.3) and undetectable median value, resp.). Subgroup analysis revealed no correlation of Th17 cells, serum IL-17, and IL-23 with disease activity or medications, nor any significant difference before and after medication. Conclusions. There is significant expansion of Th17 cells and elevated serum IL-17 and IL-23 levels in TA patients compared to healthy controls.

Highlights

Th17, γδT, Natural killer (NK), and natural killer T (NKT) cells in peripheral blood and serum IL-17 and IL-23 in Takayasu arteritis (TA) were measured and correlated with disease activity
We found an increase in serum levels of IL-17A and IL-23 in patients with TA compared with controls
We found increased expansion of Th17 cells, which produce IL17 A, but not with NK, NKT, or gamma delta T cells in the peripheral blood

Summary

Introduction

Th17, γδT, NK, and NKT cells in peripheral blood and serum IL-17 and IL-23 in Takayasu arteritis (TA) were measured and correlated with disease activity. Adaptive immunity plays a role in TA peripheral blood of patients having an increased ratio of CD4+/CD8+ T cells [2]. Studies in Giant Cell Arteritis (GCA), the other variant of LVV, have shown increased Th1 and Th17 (T-helper 17 cells, producing IL-17A) in peripheral blood and elevated serum IFN-γ and IL-17A [3]. Since the role of IL-17A has not yet been clearly defined in TA, we proposed to study cell populations that are producers of IL17A in peripheral blood, that is, Th17 cells, NK cells, NKT cells, and γδ T cells, and serum levels of IL-17A and IL-23 in patients with TA, and looked for their relationship with clinical disease activity

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