Abstract
Lymphocytes from young and old donors were incubated with PHA for 96 h and exposed to [ 3H]Tdr during the last 24 h of culture. Comparable amounts of [ 3H]Tdr were incorporated into chromosomes of old and young lymphocytes as measured by autoradiography of metaphase chromosomes. However, chromosomal damage and cell-cycle arrest were far greater in lymphocytes from old as compared to young humans. The frequency of chromosome breaks, fragments, exchange figures and dicentric chromosomes induced by [ 3H]Tdr was greater in cultures from old than in cultures from young humans. Lymphocytes from old donors exposed to 20 μM BrdU during the last 24 h of culture showed significantly more sister-chromatid exchanges than did lymphocytes from young donors. These data suggest that chromosomes in lymphocytes from old donors express more damage after exposure to [ 3H]Tdr or BrdU than do chromosomes in lymphocytes from young donors.
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