Abstract
The purpose of this study was to determine the sphingolipid (SL) profile in cells defective in autophagy protein ATG‐7 and overall cell death after photodynamic therapy (PDT) with the photosensitizer Pc 4. MCF‐7 human breast cancer cells with downregulated ATG‐7 and their scrambled controls (Scr) were used. Exposure of ATG‐7 knockdown cells to PDT led to increased cell killing. PDT evoked an early (2 h) greater global increase in ceramides in ATG‐7 defective cells compared to Scr cells. The increases in dihydroceramide (DHceramide) were distinctly higher at 2 h than at 24 h in both cell types post‐PDT. The levels of sphingosine‐1‐phosphate (S1P) and sphingosine were decreased below resting levels at 2 h irrespective of the cell type. The data imply that ceramide might be a marker of ATG‐7 deficiency in cells sensitized to PDT.
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