Abstract
Cervical cancer is the third most common cancer and the fourth leading cause of malignancy related mortality in women worldwide. CCL19 is highly expressed in human cancer cells, and ligand CCL19 binding to CCR7 induces actin polymerization and pseudopodia formation. However, whether or not CCL19 is involved in EMT of human cervical cancer needs further investigation. Using quantitative PCR and western blot analyses, we found that CCL19 is overexpressed in cervical cancer cell lines and tissues. Knockdown of CCL19 via siRNA inhibited the proliferation of cervical cancer cells by increasing apoptosis. Further analyses showed that inhibitory effects of CCL19 on cell migration and invasion were partly associated with EMT process. In conclusion, these data indicate that CCL19 is abnormally expressed in cervical cancer, indicating a novel and important role for CCL19 in cervical cancer malignant transformation.
Highlights
Cervical cancer has been generally considered as one of the most common cancers in women worldwide [1]
CCL19 was higher expressed in cervical cancer cell lines (C33A, HeLa, CaSki, SiHa, and ME-180) than in normal human cervical epithelial cell line H8 (Figure 3A)
In order to investigate the role of CCL19 in cervical cancer cell lines, three siRNA was used to knockdown CCL19 expression in ME-180 and HeLa which have the highest of CCL19 expression (Figure 3B and 3C)
Summary
Cervical cancer has been generally considered as one of the most common cancers in women worldwide [1]. Molecular alterations of tumor suppressor genes and/or oncogenes have a pivotal role in the progression of cervical cancer. Increasing evidences indicate that both chemokines and chemokine receptors play critical roles in progression of solid tumors including cervical cancer [4,5]. Previous studies show that numbers chemokines (i.e. CCL19, CXCL12) overexpressed in various tumors, which activate tumor proliferation related signaling pathway and stimulate angiogenesis on one hand, recruit immune cells infiltrating into cancerous foci and trigger tumor associated inflammation on the other [6]. Considerable studies have focused on the potential role of CCL19/ CCR7 axis in several cancers [9,10,11]. The role of CCL19 in cervical cancer has not been comprehensively studied until recently. This study highlights the emerging role of CCL19, and suggests potential target of CCL19 pathway in cervical cancer tumorigenesis
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
