Abstract
The importance of an increased amount of carbon dioxide in the air in contact with primary cultures of certain pathogenic micro-organisms has been known for a good many years. Cohen and Fleming 1 employed this method, with associated Bacillus subtilis cultures, in isolating meningococci, but regarded their success as due to the reduction in oxygen tension. Chapin 2 obtained much better growths of primary cultures of gonococci by increasing the carbon dioxide content of the air to 10 per cent. Bang 3 carried out a series of experiments on the effects of varying carbon dioxide and oxygen tensions on the growth of Brucella abortus but came to the conclusion that the latter, rather than the former, was the governing factor. A good many years later Huddleson 4 definitely established the importance of increased carbon dioxide tension for the growth of recently isolated strains of Brucella abortus, but he believed that
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