Increased biomass and reduced tissue cadmium accumulation in rice via indigenous Citrobacter sp. XT1-2-2 and its mechanisms

Abstract

Microbial remediation is a promising technique to remediate heavy metals contaminated soils. In this study, the cadmium (Cd)- resistant Citrobacter sp. XT1-2-2, isolated from heavy metals contaminated paddy soils, was investigated to evaluate the effect of this strain on soil Cd speciation, cellular Cd distribution, tissue Cd accumulation and rice biomass. The percentage of Cd2+ removal by Citrobacter sp. XT1-2-2 was up to 82.3 ± 2.1% within 240 min in the solution. The average content of soil soluble plus exchangeable and carbonate-bound fractions of Cd decreased, whereas Fe/Mn oxide-bound, organic matter-bound and residual fractions increased with bacteria inoculation. For the paddy soil inoculated with the XT1-2-2 strain, Cd concentrations of roots, culms, leaves and grains were significantly reduced by 24.1%, 46.9%, 41.5% and 66.7%, respectively. In addition, inoculation bacteria significantly increased the biomass of the roots, above-ground tissues and the rice grains. All results indicated that the XT1-2-2 strain had the ability to immobilize soil Cd and decrease Cd accumulation in rice grains. Therefore, the XT1-2-2 strain has potential for application to remediate Cd-contaminated paddy soils. It is possible to exploit a new bacterial-assisted technique for the remediation in Cd-contaminated paddy soils.