Increased Beneficial Outcome of Insulin by Medical Ozone against Experimentally Diabetic Rats

Abstract

The present research aimed to determine the effective dose of medical ozone on some blood parameters in experimental diabetic rats.48 adult female rats with (a 180 - 200 g) weight average were randomly divided into (6) groups (n=8): control (T1), Medical ozone (MO) (T2), diabetic (T3), ozone-treated diabetic (T4), insulin-treated diabetic(T5), and MO, insulin-treated diabetic (T6). Diabetes was induced by an intraperitoneal injection of a single dose of Alloxan (150 mg/kg of the body weight). The diabetic rats were left for 2 days to assure the stability of diabetes in them, before continuing experiments. The results of RBCs in groups (T2, T4, T5, and T6) showed a significant increase compared with the diabetic group and control group, and Hb and WBCs parameters showed a significant decrease in (T3, T4, and T5)When compared with other groups. The results of the platelet count there was a significant increase in (T3, T4, and T5) compared to the control group. Clotting time and calcium ions recorded a significant decrease in all treated diabetic groups (T3, T4, T5, and T6) when compared with (T1 and T2), at the same time, the results of bleeding time recorded a significant decrease in (T3 and T4 ) compared with the other groups while (T4 and T5) showed non-significant difference compared to (T1 and T2). The current study concluded that the treatment combination of medical ozone and insulin has ameliorative effects on some blood parameters except clotting time and calcium, ions.