Abstract
Synchronous fluorescence scanning at constant wavenumber (i.e., energy) difference between excitation and emission brings several improvements when compared to the classical spectrofluorimetry or to the method of synchrnous wavelength scanning. When quantitative results are required, the constant energy-difference technique should be used whenever the difference between the excitation and emission wavelengths exceeds the difference between the wavelengths of the Rayleigh and Raman peaks. Otherwise, the technique brings only minor advantages in comparison with synchronous wavelength scanning.
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