Increase of GAP‐43 in the rat cerebellum following unilateral striatal 6‐OHDA lesion

Abstract

In order to further characterize synaptic alterations following a severe lesion of the nigrostriatal system, the expression of synaptic marker proteins, synaptophysin and growth-associated protein-43 (GAP-43), was examined in various brain regions of 6-hydroxydopamine (6-OHDA)-treated rats, an animal model of Parkinson's disease. Unilateral nigrostriatal lesioning induced an increase in synaptophysin protein levels by 68% and 106% in the sensorimotor cortex and striatum, respectively, while changes in the level of GAP-43 were not observed. In contrast, 6-OHDA induced a 73% increase in the level of GAP-43 protein in the cerebellum. This increase was also confirmed with immunohistochemistry. The level of synaptophysin in the cerebellum remained unchanged in response to the lesion. These results suggest that a neurotoxic lesion of the nigrostriatal pathway differentially affects the expression of the two synaptic proteins and that plasticity-related changes in this model are not solely restricted to the nigrostriatal system. In addition, these results provide further evidence of the involvement of the cerebellum in the late response to a 6-OHDA lesion.