Abstract
We demonstrate here that stimulated45Ca2+influx in A7r5 vascular smooth muscle cells induced either by receptor activation with [Arg]8vasopressin or by the SR-Ca2+-ATPase inhibitor thapsigargin was increased more than threefold if cells were preloaded with the intracellular calcium chelator BAPTA (1,2-bis(2-aminophenoxy)ethane-N,N,N′,N′-tetraacetic acid). The increased influx is probably due to an attenuation of negative feedback of Ca2+on its own entry accompanied by increased Ca2+storage capacity of BAPTA-loaded cells leading to diminished cellular Ca2+release. We propose that BAPTA preloading could be a useful approach to investigate receptor-induced Ca2+influx.
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