Abstract
Aluminium (Al) stress increases ATP-dependent H+ transport activity of tonoplast-enriched membrane vesicles prepared from barley roots. Also, Al stress significantly increased ABA content in roots. Treatment of roots with abscisic acid (ABA), as in the case of Al stress, increased ATP-dependent H+ transport activity of tonoplastenriched membrane vesicle preparations. Vanadate treatment, which is expected to reduce the plasma membrane H+-ATPase activity as does Al stress, increased both ABA content in roots and the H+ transport activity of tonoplast membrane vesicles. Any treatment with Al, ABA and vanadate increased nitrate-inhibitible ATPase activity of prepared membrane vesicles. These results suggest that the increase of ABA in Al-stressed roots may play a key role in the activation of H+-pump of the tonoplast.
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