Abstract
The content of plasmalogens in bovine brain was investigated with respect to age. No difference between the plasmalogen content in brain of young and old individuals was detected. In old individuals, plasmalogens suffer much easier hydrolysis to corresponding aldehydes than in young ones. In addition, the brain of old animals contain an approx. 30-fold higher amount of free aldehydes and plasmalogen epoxides compared to young ones. Aldehydes, as well as derived α-hydroxyaldehydes, were trapped by addition of pentafluorobenzylaminehydrochloride. The resulting pentafluorobenzyloxime derivatives were enriched by thin-layer chromatography, transferred to trimethylsilyl derivatives and further investigated by GC/MS. Quantification was achieved by GC/FID as well as by GC/ECD.
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