Increase in the number of intrafusal muscle fibres in rat muscles after neonatal motor denervation

Abstract

Rat muscle spindles disintegrate after total neonatal muscle denervation in which both sensory and motor axons are cut, but develop almost normally during the first three weeks after neonatal de-efferentation, attaining the usual complement of four intrafusal fibres, two bag and two chain fibres. Thereafter additional intrafusal fibres differentiate in most of the de-efferented spindles. We have examined the development of supernumerary fibres in muscle spindles of the rat hind limb muscles one to four months after neonatal de-efferentation using transmission electron microscopy. Additional intrafusal fibres originate both from activated intrafusal myosatellites and by different forms of fibre division. In a sample of 27 muscle spindles examined along the A zone five weeks after de-efferentation, the mean number of intrafusal fibre profiles increased almost two-fold to 7.9 ± 3.3 (S.D.). Up to 20 intrafusal fibre profiles per spindle cross-section were found in muscles de-efferented for four months. The supernumerary fibres were apparently derived from all three intrafusal fibre types, but predominantly from the chain fibres. It is noteworthy that de-efferentation which causes wasting of extrafusal muscle initiates myogenesis and maintains additional intrafusal fibres within the spindle capsules.