Abstract
Dry seed lots of four rice varieties with different heading dates were exposed to thermal neutrons and to gamma-rays, with and without boron-enrichment pre-treatment of seed. M 1 plants were grown and their seeds were separately harvested and examined for frequencies of chlorophyll, heading date, and sterile mutations in the M 2 generation. The frequencies of chlorophyll mutations were not significantly different among the treatments. The frequencies of heading date mutations tended to be higher in the thermal neutron treatment with boron-enrichment than that without boron-enrichment. For the gamma-ray treatment, the opposite applied to the frequency of sterile mutations. The practical implication of boron-enrichment pre-treatment of seed in the use of thermal neutrons for mutation breeding of crop plants is discussed.
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