Abstract
Incubation of acetaldehyde with hemoglobin has been shown by chromatographic methods to result in an increase in fast hemoglobin. We have studied this increase in fast hemoglobin concentration by a gel electrophoretic method. Increases in fast hemoglobin concentration are detected following incubation with concentrations of 100 microM acetaldehyde and no further increase in yield occurs with concentrations greater than 7 mM acetaldehyde. The reaction is complete within 5 hr and the product is stable with a half-life of greater than 12 days.
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