Increase in antigen-induced release of slow reacting substance of anaphylaxis from guinea pig lung by sodium fluoride

Abstract

Some of the mechanisms involved in the release of mediators of anaphylaxis from passively sensitized guinea pig lung were examined. NaF (10 mM) markedly enhanced antigen-induced release of slow reacting substance of anaphylaxis (SRS-A), whereas release of histamine was not changed. Similar results were obtained with equimolar amounts of KF but not with NaCl. When SRS-A, released from lung in the presence of NaF, was applied to guinea pig ileum, the tissue contracted. This response was selectively antagonized by FPL 55712, an SRS-A antagonist. A23187, a divalent cation ionophore, released histamine and SRS from guinea pig lung. NaF and KF, but not NaCl, reduced equally the amounts of mediators released by A23187. These results indicate that antigen-induced release of histamine and SRS-A can be dissociated by fluoride ions. Furthermore, they suggest that NaF may prove useful in dissecting and understanding some of the complex biochemical steps involved in the release of SRS-A.