Incorporation of Nano Selenium in Fish Diet and Assessment of Growth Performance and Biochemical Criteria of Labeo rohita

Abstract

Selenium (Se) is an essential microelement utilized in aqua-feeds for aquatic animals’ normal growth, well-being and health. This work was intended to assess the growth performance of Labeo rohita (Rohu) resulting from the nano Se-merged diet. Nano Se was synthesized and its physico-chemical characteristics were characterized using UV-VIS, SEM, EDAX, XRD and FTIR for varying quantities of nano Se; Diets - I-0, II- 0.5, III-1, IV-1.5, V-2 and VI- 2.5 mg/ kg were prepared, making use of fish meal (FM), groundnut oil cake (GNOC), wheat flour (WF) and tapioca flour (TF). Feed-utilizing parameters and the biochemical composition of Rohu were evaluated subsequently after 28 days. The UV-Vis Spectroscopy revealed that nano Se was assessed in wavelengths of 200 to 500 nm and exhibited strong absorption at 322 nm. SEM image showed spherical morphology with an average particle diameter of 12.22 mm. EDAX spectrum recorded two signals at 1.5 keV and 11 keV. XRD patterns showed crystalline characteristics of nano Se at 2θ correlators of 23.5o, 29.7o, 41.4o, 43.6o, 45.4o, 51.7o, 55.9o and 61.5o. FTIR spectrum was examined in the range of 4000 – 500 cm-1. Rohu's growth performance and biochemical analysis revealed that the protein, carbohydrate and lipids of gill, liver and muscle of Rohu were highly increased in the case of diet IV containing 1.5 mg/kg nano Se.