Abstract
Addition of proline to suspensions of nonpigmented, nonproliferating cells of Serratia marcescens induced biosynthesis of the pigment, prodigiosin. If methionine was included with proline, 4 times as much prodigiosin was formed, although the amount synthesized in the presence of methionine alone was nil. Uniformly 14C-labelled proline and methionine were incorporated into prodigiosin to about 30% the extent of their incorporation into cellular protein. Experiments with [ carboxy- 14C]-, and [ Me- 14C] methionine established that isotope from the methyl group was utilized preferentially for biosynthesis of prodigiosin.
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