Abstract
Background13-Methyltetradecanoic acid (13-MTD), an iso-C15 branched- chain saturated fatty acid, has been shown to induce apoptotic cell death of numerous human cancer cells. However, the mechanism for the induction of apoptosis has not been fully understood. This study described the incorporation of 13-MTD into cellular lipid of SKBR-3 breast cancer cells and apoptosis related event to gain more insight into the mechanism action of this fatty acid.ResultsTreatment of SKBR-3 cells with 13-MTD lowered the cell viability and induced apoptosis. Proportion of 13-MTD in the glycerolipids increased to saturation level within 6 hours. Triacylglycerol contained 13-MTD in higher concentration than phospholipid with positional preference to sn-2. 13-MTD caused no changes in the caspase activity and its gene expression. Furthermore, addition of caspase-inhibitor to culture medium did not prevent the cells from the cytotoxicity of 13-MTD. No-increase in the cellular calcium level was also noted with 13-MTD treatment. However, 13-MTD disrupted the mitochondrial integrity in 4 hours, and increased the nuclear translocation of apoptosis inducing factor.ConclusionThese results showed that 13-MTD disrupted the mitochondrial integrity, and induced apoptosis via caspase-independent death pathway.
Highlights
The branched-chain fatty acid (BCFA) or alcohols has been reported to suppress the proliferation or development of tumor cells [3]
The effect of 13-methyltetradecanoic acid (13-MTD) on the relative viability of SKBR-3 cells are shown in figure 1
Consistent with the previous results, 13-MTD lowered the cell viability compared with the straight chain fatty acid counterpart, but to a lesser extent than cis-9, trans-11 conjugated linoleic acid (CLA), a well known anticancer fatty acid
Summary
The branched-chain fatty acid (BCFA) or alcohols has been reported to suppress the proliferation or development of tumor cells [3]. 13-methyltetradecanoic acid (13-MTD), an iso-C15 saturated fatty acid, was purified as an anti-cancer agent from a soy-fermentation prod-. Relative cell viability (%) Percentage of apoptotic cells (%). EFfifgeuctreof113-MTD on the relative viability of SKBR-3 cells Effect of 13-MTD on the relative viability of SKBR-3 cells. Cells were treated with the fatty acid samples for 24 h, and the relative viabilities were determined by MTS assay kit according to the manufacture's instruction. Data are mean ± SE of 8 analyses. Data not sharing the same letter within each concentration are significantly different at p < 0.05 by TukeyKramer test
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