Incorporation of arachidonic and linoleic acid hydroperoxides into cultured human umbilical vein endothelial cells

Abstract

The current study assessed the differential incorporation of 12-hydroperoxyeicosatetraenoic acid (12-HPETE), arachidonic acid (AA), 12-hydroxyeicosatetraenoic acid (12-HETE) and the linoleic acid (LA) oxidation products, 13-hydroxyoctadecadienoic acid (13-HODE) and 13-hydroperoxyoctadecadienoic acid (13-HPODE), into human umbilical vein endothelial cells (HUVEC). Approximately 80–90% of AA (10 −8–10 −5 M) and 80% of LA (10 −8–10 −5 M) were incorporated into HUVEC within 12 h, while less than 50% of the hydroxy metabolites (12-HETE, 12-HPETE, 13-HODE, 13-HPODE) were incorporated into HUVEC over 48 h. Further, treatment of HUVEC with either 12-HPETE or 13-HPODE (concentrations of 10 −5 M) had no effect on cell number at a 48 h time point when compared with control. These results demonstrate that exogeneous hydroxy metabolites are incorporated into HUVEC to a lesser degree than were endogenous fatty acids. Further, we speculate that 12-HPETE and 13-HPODE are rapidly metabolized to substances without significant cytotoxic effects.