Abstract
Purified rat ventral prostate nuclei, lacking the outer layer of their nuclear envelopes, incorporated radioactive amino acids into hot trichloroacetic acid-insoluble materials. Some properties of this reaction examined include: kinetics, pH and temperature dependence, effects of possible inhibitors, and of changes in concentration of nuclei or radioisotope. Association of radioactivity with incubated nuclei was demonstrated by light microscope autoradiography. Labelled proteins extracted from prostate nuclei and microsomes and examined by polyacrylamide gel electrophoresis yielded dissimilar patterns. This limited incorporation, catalyzed by prostate nuclei, resembles that described with rat thymus, liver and HeLa cell nuclei. While its biochemical role as reflected by assay in vitro is obscure, administration of androgen restored the reduced activity that followed castration; the process is partially androgen-dependent.
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