Abstract
Adult Rhipicephalus appendiculatus ticks, infected with Theileria parva, were stimulated by rabbit-feeding for 0–5 days and then exposed to 3H-hypoxanthine while being maintained in vitro as backless ticks. Autoradiography showed that most incorporation of 3H-hypoxanthine occurred during the first four days after stimulation, when T. parva parasites were actively proliferating. DNA and RNA were extracted from infected and uninfected salivary glands, and incorporation of radio-label was measured by liquid scintillation counting. There was higher incorporation into nucleic acids of Theileria-infected salivary glands than of uninfected salivary glands, and incorporation into DNA was only observed in infected salivary glands. The current work suggests that the period of greatest nucleic acid synthesis precedes the period during which most of the infected sporozoites are secreted.
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