Incorporation of [1-14C]-Isopentenyl Pyrophosphate into Monoterpenes by a Cell-Free Homogenate Prepared from Callus Cultures ofChrysanthemum cinerariaefolium

Abstract

Callus culture of CHRYSANTHEMUM CINERARIAEFOLIUM (Trev.) Bocc. (Asteraceae), initiated with axillary buds from the plant, have been shown to produce chrysanthemyl alcohol and chrysanthemic acid. Incubation of a cell-free homogenate from the callus with [1- (14)C]-isopentenyl pyrophosphate demonstrated incorporation of radioactivity into chrysanthemyl alcohol and chrysanthemic acid as well as products from isopentenyl pyrophosphate isomerase (EC 5.3.3.2; isopentenyl diphosphate Delta (3)-Delta (2)-isomerase) and prenyltransferase activities (EC 2.5.1.11; allylic-terpene-diphosphate:isopentenyl diphosphate terpenoid-allyltransferase).