Incomplete Multi-view Clustering for Single cell RNA Sequencing Data

Abstract

With the rapid development of sequencing technology, researchers can obtain a large number of single cell RNA sequencing (scRNA-seq) data which is useful for analysis of cell fate decision and growth process at individual cell resolution. But due to the limitations of sequencing technology, the data acquired has dropouts which may affect the results of down-steam analysis. Therefore, many algorithms have been proposed to impute the data before clustering, here in, imputation and clustering are considered as two separate processing stage. In this paper, we adopt a clustering algorithm—Incomplete Multiple Kernel k-means Clustering with Mutual Kernel Completion (MKKM-IK-MKC) to analyze scRNA-seq data. It unifies imputation and clustering into a process. Comparing with some existing "two stage" (imputation +clustering) algorithms, the experimental results on five scRNA-seq datasets from various species demonstrate the effective performance of our new proposed method.