Incoherent digital holographic microscopy for high-speed three-dimensional motion-picture sensing

Abstract

We present incoherent digital holographic microscopy systems for high-speed three-dimensional (3D) motion-picture sensing. Adopting single-shot phase-shifting interferometry to incoherent digital holography, high-speed motion-picture recording of spatially incoherent digital holograms can be enabled. Single-path self-interference incoherent digital holography systems are designed and constructed utilizing the polarization of light waves and birefringent optical elements. We describe the systems constructed until now and show experimental results including three-dimensional motion-picture recording of incoherent digital holograms for fluorescent nanoparticles in a flow, at the frame rate of more than 100 fps and with 1024 x 1024 pixels and the field of view of 148 um x 148 um. Furthermore, we have succeeded in extending the applicability of incoherent digital holography to 3D sensing of objects with compact optical systems. In the video presentation, single-shot quantitative phase imaging with a light-emitting diode will be introduced.