Abstract
The inclusion of fluorescein (FL), erythrosin B (EB), and rose bengal (RB) in α-, β-, γ-cyclodextrins (α-, β-, and γ-CDs) was studied with several techniques. Induced circular dichroism (icd) absorption spectroscopy and steady-state and picosecond time resolved fluorescence spectroscopy were used to derive stoichiometry, association constants, and structural information on the inclusion complexes formed. A stoichiometry of 1:1 was found for the range of concentration explored (xanthenes, 10 -6 -10 -4 M; CD, 10 -3 -10 -2 M). From icd measurements, FL is found to bind weakly with α- and γ-CDs (K A =35 M -1 ) and associate preferably with β-CD (K A =360 M -1 )
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