Abstract
Complexation with cyclodextrins (CDs) is a technique that has been extensively used to increase the aqueous solubility of oils and improve their stability. In addition, this technique has been used to convert oils into solid materials. This work aims to develop inclusion complexes of Copaifera multijuga oleoresin (CMO), which presents anti-inflammatory activity, with β-cyclodextrin (β-CD) and hydroxypropyl-β-cyclodextrin (HP-β-CD) by kneading (KND) and slurry (SL) methods. Physicochemical characterization was performed to verify the occurrence of interactions between CMO and the cyclodextrins. Carrageenan-induced hind paw edema in mice was carried out to evaluate the anti-inflammatory activity of CMO alone as well as complexed with CDs. Physicochemical characterization confirmed the formation of inclusion complex of CMO with both β-CD and HP-β-CD by KND and SL methods. Carrageenan-induced paw edema test showed that the anti-inflammatory activity of CMO was maintained after complexation with β-CD and HP-β-CD, where they were able to decrease the levels of nitrite and myeloperoxidase. In conclusion, this study showed that it is possible to produce inclusion complexes of CMO with CDs by KND and SL methods without any change in CMO’s anti-inflammatory activity.
Highlights
Copaifera multijuga Hayne (Leguminosae) is a tropical tree commonly found in Brazil, in the central and western region of Amazon
Previous reports have shown that copaiba oil and its main sesquiterpene have some biological activities such as anti-inflammatory [6], antimicrobial [7,8,9], anti-carcinogenic [10], anxiolytic, anti-depressant [11,12] and analgesic activities [13]
The peak assigned to the chemical marker was confirmed by comparing the retention time of the standard (18.24 min) with that of the sample
Summary
Copaifera multijuga Hayne (Leguminosae) is a tropical tree commonly found in Brazil, in the central and western region of Amazon. Among the forms of oleoresins extraction, tapping the trunk of Copaifera multijuga Hayne is currently the only method that does not cause the death of the tree. The genus Copaifera L. has been studied due to its several biological activities, where the anti-inflammatory effect is the most investigated [1,2,3,4]. Copaiba oil is in an oleoresin that consists of an exudate of acidic resins and volatile compounds [3]. The latter are usually sesquiterpenes, of which β-caryophyllene is the most abundant compound found in the oleoresin [5]. Its anti-inflammatory activity is attributed to the synergism of several compounds present in the oleoresin [4,14]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
