Abstract
Two different populations of vacuoles were released from protoplasts of isolated Acer pseudoplatanus cells by ultracentrifugation on density gradient. It was shown by fluorescence microscopy and assays with marker enzymes that there were only traces of contamination by other organelles and by cytoplasmic components in the vacuole preparations. The vacuoles contained an ATPase with an optimum activity at pH 7.5. This vacuolar ATPase activity was much higher than the one of vacuoles isolated by the lysis of protoplasts with polybases. In fact, the ATPase activity of the vacuoles isolated by ultracentrifugation was inhibited by diethylaminoethyl dextran (DEAE-dextran) and dextran sulfate.
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