Incidence of multidrug resistance gene 1 3435C/T single nucleotide polymorphism in patients with amlodipine induced gingival enlargement – A case control study

Abstract

BackgroundPeriodontal diseases are multifactorial in nature with genetic cause as one of the risk factors. In current era, periodontal researches have widely expanded to explore the genetic background of periodontal diseases. Also, genetic predisposition remains as one of the causes for Drug-induced Gingival enlargement. ObjectiveTo identify MultiDrug Resistance gene (MDR) 1 C3435T single nucleotide polymorphism (SNP) in patients under amlodipine therapy with and without gingival enlargement. MethodsAbout 50 hypertensive patients were selected. They were divided into two groups; in that, 25 hypertensive subjects under amlodipine therapy with drug-induced gingival enlargement and 25 hypertensive subjects under amlodipine therapy with healthy gingiva were included as study and control group respectively. The blood Samples were collected from both groups for analyzing genotype distribution and finding the association of C3435T SNP with drug-induced gingival enlargement by PCR method. ResultsOur present study demonstrated no significant difference between genotypes and severity of gingival enlargement as the p value was less than significance value of 0.05. Therefore, the study concludes there is no association of gingival enlargement with specific genotype. ConclusionWithin the limits of the present study, it can be concluded that the susceptibility for drug-induced gingival overgrowth is not influenced by the MDR 1 C3435T single nucleotide polymorphism. Since the study was undertaken in a small sample size, definite conclusion cannot be drawn. Hence, further genetic studies in MDR 1 gene in a large population are necessary for justifying the genetic predisposition to drug-induced gingival overgrowth.