Abstract
Occurrence and incidence of drug resistant bacteria are becoming very high and common with overuse of antibiotics. This study focused on the isolation of drug resistant strains from the clinical and environmental sample that produce extended spectrum β-lactamase (ESBL) and identification of TEM-1 genes in the plasmid and genomic DNA. 45 Clinical samples from the hospital and 7 environmental samples from the polluted water sources were collected. Among the collected samples, E. coli showed a higher incidence (36.1 %) followed by Klebsiella sp., Staphylococcus sp., Streptococcus sp., Pseudomonas sp., Proteus sp., and Salmonella sp. Antibiotic susceptibility of the isolates were investigated against 25 commercially available antibiotics. All the isolated strains showed MAR index value of more than 0.2. Among the 36 isolates, 7 Gram negative isolates (19.4 %) showed positive results for ESBL production in Double disk synergy test. The plasmid and genomic DNA were isolated and analyzed using PCR with specific primers for the presence of the TEM-1 gene (716 bp). A selected PCR products of the TEM-1 gene was sequenced and analyzed using BLAST.Int. J. Appl. Sci. Biotechnol. Vol 5(3): 302-308
Highlights
Drug resistant bacteria are emerging worldwide as a threat to common infections in community and hospital settings
Beta lactamase produced by several Gram negative and Gram positive bacteria are responsible for their resistance to β-lactam antibiotics such as penicillins, cephalosporins, cephamycins, etc. β-lactamases is one of the leading causes of resistance to β-lactam antibiotics in Gram negative bacteria (Sibhghatulla et al, 2015)
ESBL producers were found to be present in polluted water bodies which reveal the transfer of their resistance to other coliforms
Summary
Drug resistant bacteria are emerging worldwide as a threat to common infections in community and hospital settings. Beta lactamase produced by several Gram negative and Gram positive bacteria are responsible for their resistance to β-lactam antibiotics such as penicillins, cephalosporins, cephamycins, etc. In the past 15 years, the extensive and sometimes abusive clinical utilization of these drugs have been responsible for the development of an increasing number of resistant strains (Coyette et al, 1994) In most cases, this be attributed to the production of new Extended Spectrum β-lactamase (ESBL) which are mediated by plasmids conferring resistance to cefotaxime, ceftazidime, and aztreonam. TEM-1 β -lactamase one of the leading cause of ampicillin resistance in 90% of E. coli (Livermore, 1995) These enzymes found in nonEnterobacteriaceae gram-negative bacteria (Mugnier et al, 1996). The present investigation is focused to reveal the incidence of multi-drug resistant bacteria in clinical and environmental samples collected from Coimbatore, India and to study beta lactamase production and to analyze the diversity of TEM-1 using molecular markers
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: International Journal of Applied Sciences and Biotechnology
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
