Abstract
Staphylococcus aureus infection remains an increasing problem for higher morbidity and mortality in burn patients. We sought to determine the frequency of methicillin-resistant S. aureus (MRSA) in burn wound patients and study their drug resistance genes. Samples were collected (August 2010 to October 2011) from burn unit of Dhaka Medical College Hospital (DMCH), Bangladesh. MRSA was identified by conventional culture based methods. S. aureus was confirmed in 44.44% burn wound samples and 22.5% of the isolates were oxacillin resistant. All the S. aureus isolates were resistant to commonly used drugs like amoxicillin, azactam, erythromycin, azithromycin etc. and were sensitive to imipenem. The isolates were devoid of plasmid and the gene mecA, femA and IS431 were detected in their chromosomal DNA. Computational analysis of mecA gene sequence showed homology to S. aureus “penicillin binding protein 2a” (PBP-2a). The higher association of MRSA is in burn unit of DMCH, Bangladesh is alarming and with inappropriate antibiotic use, and the situation gets even complicated to treat. Therefore, the detection system and control practices for MRSA in DMCH should be improved in the hospital settings.
Highlights
Burn related diseases were among the top ten diseases according to the number of patients admitted in the Dhaka Medical College Hospital during 2011 in Bangladesh [1]
Methicillin-resistant Staphylococcus aureus (MRSA) is called oxacillin resistant S. aureus (ORSA) which has long been associated as a major agent of nosocomial infection and is a prominent reason for higher morbidity and mortality in burn patients causing a variety of infections such as bacteremia, pneumonia, septic arthritis, endocarditis, and surgical site infections [6,7,8]
Samples were collected during August, 2010 to October, 2011 from burn wound patients admitted to the burn unit of Dhaka Medical College Hospital (DMCH), Dhaka1000, Bangladesh
Summary
Burn related diseases were among the top ten diseases according to the number of patients admitted in the Dhaka Medical College Hospital during 2011 in Bangladesh [1]. Burn patients become susceptible to increased rate of infection due to the loss of protective skin barrier and decreased cellular and humoral immunity [2,3]. Though the infection may be initiated by the skin flora, after entry of burn patients in a hospital nosocomial infection may initiate. Methicillin-resistant Staphylococcus aureus (MRSA) is called oxacillin resistant S. aureus (ORSA) which has long been associated as a major agent of nosocomial infection and is a prominent reason for higher morbidity and mortality in burn patients causing a variety of infec-. Data from the sentry antimicrobial surveillance program showed increasing rates of MRSA among S. aureus isolated from intensive care unit patients worldwide [13]. The genome of MRSA bears a 21 to 67 kb staphylococcal cassette chromosome mec (SCCmec) containing the mecA gene
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