Abstract
Introduction: Annual incidence rates for oral and pharyngeal cancer are estimated at 25 cases per 100,000 in developing countries. Human papilloma virus (HPV) was implicated in pathogenesis of Cancer. The mutations of p53 and c-myc are found 50% in cancer. Objective: Aims of this research were to know the incidence of OSSC patient which realized HPV infection without p53 and c-myc gene mutation. Materials and Methods: Tissue biopsy frozen sections were taken from BOSC (Benign Oral Squamous Cell) and OSCC (Oral Squamous Cell Carcinoma) patients collected from Oral and Dental Departement of dr Muwardi Distric Hospital in Surakarta from January 2007 to January 2008. To amplify L1-HPV gene for fixed the HPV stressor. To amplify p53 and c-myc genes, continued with SSCP (Single Strand Conformational Polymorphisme) analysis and followed with measurement using densitometer, to see mutation existence. The collected data were analyzed with Chi Square. Results: BOSC patient identified 23% with HPV infections and OSCC patient identified 73% with HPV infections. Hundred percent BOSC patient with HPV infection without mutation in p53 gene and c-myc gene, 81% OSCC patient with HPV infection without mutation in p53 gene and 91% OSCC patient with HPV infection without mutation in c-myc gene. Chi square analysis showed significant difference between BOSC and OSCC patients with HPV infection without mutation in p53 and c-myc gene. Conclusion: HPV is a factor for pathogenesis of OSCC. DOI: 10.14693/jdi.v17i2.44
Highlights
Annual incidence rates for oral and pharyngeal cancer are estimated at 25 cases per 100,000 in developing countries
The followed factor is periodontal disease chronic, bed oral hygiene, diseases of tooth, sharp of set teeth, electrogalvanism and edentulism. Another researcher found that human papilloma virus (HPV), implicated in Oral Squamous Cell Carcinoma (OSCC) pathogenesis.[3,6,7]
Cutting II was subjected to Deoxyribonucleic Acid (DNA) isolation was made by Henk Schmits method with some modifications
Summary
Annual incidence rates for oral and pharyngeal cancer are estimated at 25 cases per 100,000 in developing countries. Chi Square analysis showed significant difference between BOSC and OSCC patients with HPV infection without mutation in p53 and c-myc gene. Environmental factors include: environmental pollutants, diet and obesity, tobacco, infections, radiation, and lack of physical activity These environmental factors cause or enhance abnormalities in the genetic of cells.[3] The prevalence of oral cancer is increase in Africa. The followed factor is periodontal disease chronic, bed oral hygiene, diseases of tooth, sharp of set teeth, electrogalvanism and edentulism Another researcher found that human papilloma virus (HPV), implicated in Oral Squamous Cell Carcinoma (OSCC) pathogenesis.[3,6,7] if the pathogenesis of OSCC can’t be explained so the morbidity and mortality can increase and lead to decrease the human resources
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