Abstract
Clostridium perfringens was detected in 322 (74.2%) fecal specimens heated at 75°C for 20min and in 76 (17.5%) fecal specimens heated at 100°C for 60min, out of 434 specimens from healthy persons in Hokkaido. Enterotoxigenic C. perfringens was found in only 20 (4.6%) of the fecal specimens. The enterotoxigenic strain was isolated from 12 fecal specimens heated at 75°C for 20min and from 9 fecal specimens heated at 100°C for 60min, although it was isolated from one specimen after both of the heat treatments. A total of 936 C. perfringens strains were isolated from fecal specimens heated at 75°C for 20min and 645 strains of the total produced a sufficient number of spores in DS medium. Of the total (645), only 23 strains (3.5%) were enterotoxigenic, of which 15 strains produced heat-sensitive spores (A-type) and the remaining 8 strains produced heat-resistant spores (K-type). On the other hand, a total of 149 C. perfringens strains were isolated from fecal specimens heated at 100°C for 60min and 104 strains of the total produced large numbers of spores in DS medium. Of the total (104), only 16 strains (15.4%) were enterotoxigenic and produced heat-resistant spores (K-type) only.
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