In-cell NMR based technology to study protein interactions

Abstract

High-resolution structural studies of proteins and protein complexes in a native prokaryotic and eukaryotic environment present a challenge to structural biology. In-cell NMR can characterize atomic resolution structures but requires high concentrations of labeled proteins to be delivered into intact cells. Most exogenous delivery techniques are either limited to specific cell types or are too destructive to preserve cellular physiology. We demonstrate the feasibility of microfluidics transfection or volume exchange for the convective transfer, VECT, as a means to deliver labeled targets to HeLa cells.