Inactivation of tomato pectic enzymes by manothermosonication

Abstract

The resistance of tomato pectic enzymes to manothermosonication (MTS), a combined treatment of heat and ultrasound under moderate pressure, was studied. Pectinmethylesterase (PMF) and polygalacturonases (PG) I and II were inactivated much more efficiently by MTS than by simple heating. In MTS inactivation of these enzymes, the effect of heat and ultrasonic waves was synergistic. D values [the time required for the (original) enzyme activity to decrease by 90%] for PME heat inactivation at 62.5 °C were reduced 52.9-fold by MTS and those for PG I at 86 °C and PG II at 52.5 °C, 85.8-fold and 26.3-fold, respectively.