Inactivation of Staphylococcus aureus by the synergistic action of charged and reactive plasma particles

Abstract

In this paper, a low-pressure capacitively coupled plasma discharge sustained in an argon-oxygen mixture was studied in order to evaluate its properties in terms of inactivation of Staphylococcus aureus. The plasma parameters as electron temperature and plasma density were measured by the Langmuir probe (Ne ≈ 1015 m−3, Te ≈ 1.5 eV), while the neutral atom density was in the range of 1021 m−3. In the plasma phase, oxygen radicals were taken as reference of the reactive species with antimicrobial activity, and oxygen spectral lines, over a range of plasma process parameters, were investigated by the optical emission spectroscopy. Optimal plasma conditions were found, and a count reduction of 4 log in a few minutes of the bacterium proves the potentiality of an industrial grade plasma reactor as a sterilization agent.