Inactivation of Salmonella Enteritidis PT30 on the surface of unpeeled almonds by cold plasma

Abstract

The contamination of nut products, like almonds, with human pathogens is a reoccurring concern in the food industry. In this study the inactivation of Salmonella Enteritidis PT 30 (ATCC BAA-1045) on the surface of unpeeled almonds by cold atmospheric pressure plasma was investigated. Air, O2, N2, CO2 and 90% CO2 + 10% Ar were used as process gas. Inoculum preparation and inoculation of almonds was done according to the guidelines recommended by the Almond Board of California. Furthermore, impact of plasma treatment on product color was measured. All plasma treatments inactivated Salmonella. Maximum achieved inactivation depends on the used process gas. Air plasma inactivated > 5.0 log10, O2 plasma 4.8 and N2 plasma 2.0 log10 after 15 min treatment. The plasma treatment with air and N2 resulted in a browning of the unpeeled almond's surface color. Whereas the other used plasma did not alter the color considerably. The contamination of raw nuts with human pathogens is an on-going food safety concern. The application of cold atmospheric pressure plasma has a high potential as a gentle technology for the surface decontamination. The results of this study suggest that a cold plasma treatment could be an alternative technology for the pasteurization of almonds. The use of air plasma achieved more than a 5 log10, which is in general required by the U.S. Food and Drug Administration for the approval as an alternative inactivation technology for food products. However, the scale up to commercial treatment levels requires the complete understanding of the involved product-plasma interactions.