Abstract
Surface disinfection is part of a larger mitigation strategy to prevent the spread of the severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) virus causing coronavirus disease-2019 (COVID-19). Research evaluating the time, nature, and extent of surface disinfection of replication-competent viruses is needed. We evaluated the efficacy of two disinfectants against a replication-competent SARS-CoV-2 surrogate on three common public surfaces. Vesicular stomatitis virus expressing green fluorescent protein (VSV-GFP) was our replication-competent SARS-CoV-2 surrogate. Disinfection occurred using Super Sani-Cloth Germicidal Disposable Wipes and Oxivir Tb spray per manufacturer instructions to test the efficacy at reducing the presence, viability, and later replication of VSV-GFP on stainless steel, laminate wood, and porcelain surfaces using standardized methods after recovery and toxicity testing. During the main trials, we placed 100 µL spots of VSV-GFP at viral titers of 108, 107, and 106 PFU/mL on each surface prior to disinfection. Trials were completed in triplicate and post-disinfection measurements on each surface were compared to the measurements of non-disinfected surfaces. Disinfectants were considered efficacious when ≥3-log10 reduction in the number of infectious VSV-GFP virus units was observed on a given surface during all trials. Both disinfectants produced a ≥3.23-log10 reduction in infectious VSV-GFP virus unit numbers, with all trials showing no viable, replication-competent VSV-GFP present on any tested surface. The two disinfectants eliminated the presence, viability, and later replication of VSV-GFP, our SARS-CoV-2 surrogate, on all surfaces. This information suggests that, if following manufacturer instructions, overcleaning surfaces with multiple disinfectant solutions may be unnecessary.
Highlights
Introduction iationsSevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the virus causing coronavirus disease 2019 (COVID-19), has resulted in high morbidity and mortality [1].Evidence that most COVID-19 infections are transmitted by asymptomatic individuals [2,3]highlights the continued need for effective SARS-CoV-2 prevention and control measures.given the time needed to distribute and administer approved vaccines at a global level, reducing SARS-CoV-2 transmission in public spaces continues to be crucial, with this research being important for the control of seasonal respiratory illnesses such as influenza.The Centers for Disease Control and Prevention (CDC) makes hazard control recommendations based on the Hierarchy of Controls [4]
The raw plaque count data and the average PFU/mL of virus recovered on each surface type with and without disinfection are shown in Tables 1–3 for stainless steel, laminate wood, and porcelain, respectively
Germicidal Disposable Wipe or Oxivir Tb when used per manufacturer recommendations. Disinfection by both disinfectants at each multiplicity of infection (MOI) on all surface types contributed to ≥3-log10 reduction in viable Vesicular stomatitis virus expressing green fluorescent protein (VSV-GFP) virus
Summary
Introduction iationsSevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the virus causing coronavirus disease 2019 (COVID-19), has resulted in high morbidity and mortality [1].Evidence that most COVID-19 infections are transmitted by asymptomatic individuals [2,3]highlights the continued need for effective SARS-CoV-2 prevention and control measures.given the time needed to distribute and administer approved vaccines at a global level, reducing SARS-CoV-2 transmission in public spaces continues to be crucial, with this research being important for the control of seasonal respiratory illnesses such as influenza.The Centers for Disease Control and Prevention (CDC) makes hazard control recommendations based on the Hierarchy of Controls [4]. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the virus causing coronavirus disease 2019 (COVID-19), has resulted in high morbidity and mortality [1]. Highlights the continued need for effective SARS-CoV-2 prevention and control measures. Given the time needed to distribute and administer approved vaccines at a global level, reducing SARS-CoV-2 transmission in public spaces continues to be crucial, with this research being important for the control of seasonal respiratory illnesses such as influenza. The Centers for Disease Control and Prevention (CDC) makes hazard control recommendations based on the Hierarchy of Controls [4]. This Hierarchy posits that the most effective hazard control measure is removal of the environmental hazard rather than relying.
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