Abstract
Acrolein, a highly reactive aldehyde found in cigarette smoke, was shown to induce time-dependent inactivation of NAD +-linked 15-hydroxyprostaglandin dehydrogenase from porcine lung. The inactivation process followed pseudo-first-order kinetics and was irreversible. Inactivation by acrolein can be prevented by prior incubation of the enzyme with GSH but not by subsequent addition of GSH during assay. Inactivation can be also protected fully by prior incubation of the enzyme with NAD +, but only partially with prostaglandin E 1. The results suggest that acrolein alkylates the enzyme at the coenzyme binding site and inactivates the enzyme. Inactivation of pulmonary NAD +-linked 15-hydroxyprostaglandin dehydrogenase by acrolein may alter cellular and circulating thromboxane/prostacyclin ratios and subsequently disturb vascular homeostasis and augment inflammatory and anaphylactic responses in smokers.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
