Year-end Sale % OFF 
Abstract
Human papillomaviruses (HPV) are non-enveloped DNA viruses infecting cutaneous and mucosal squamous epithelia. Sexually transmitted HPV-types that are carcinogenic to humans such as HPV16 can induce cervical and other anogenital cancers. Virus transmission through fomites such as inadequately disinfected gynecological equipment is a further potential transmission route. Since HPV cannot be easily grown in cell culture, polyomavirus SV40 has been used as a surrogate virus when testing the virucidal activity of chemical disinfectants. So far, studies that have compared the virucidal activity of different disinfectants against HPV and SV40 are lacking. Here, we evaluated the susceptibility of HPV16 pseudovirus and SV40 to seven active biocidal substances using quantitative suspension tests. Ethanol, glutaraldehyde (GTA), dodecyldipropylentriamin (DPTA), and ortho-phthalaldehydes (OPA) were able to reduce the infectivity of HPV16 pseudovirus >99.99% after 5 min. In contrast, isopropanol, peracetic acid (PAA), and quaternary ammonium compounds with alkylamines (QAC) only led to a slight or no reduction in infectivity. Concerning SV40, only GTA (60 min contact time), PAA, and OPA had virus-inactivating effects. In conclusion, the virucidal activity of three out of seven disinfectants tested was different for HPV16 pseudovirus and SV40. In this study, SV40 was shown to be a reliable surrogate virus for HPV when testing isopropanol-, GTA-, QAC-, and OPA-based disinfectants.
Highlights
Anogenital human papillomaviruses (HPV) are transmitted by sexual contact and HPV in risk group 1, which are carcinogenic to humans, can cause high-grade cervical, vaginal, vulvar, and anal intraepithelial neoplasia and the respective anogenital cancers [1,2,3]
We evaluated the susceptibility of HPV16 pseudovirus and simian virus 40 (SV40) to different disinfectants in order to determine whether SV40 is an appropriate surrogate virus for HPV in the context of studies on chemical disinfectants
Ethanol (60% and 70%, 5 min), DPTA 0.5% (5 min and 60 min), and GTA 0.05% at a contact time of 5 min were only effective against HPV16 pseudovirus, while peracetic acid (PAA) 0.05% at a contact time of 5 min only inactivated SV40
Summary
Anogenital human papillomaviruses (HPV) are transmitted by sexual contact and HPV in risk group 1, which are carcinogenic to humans, can cause high-grade cervical, vaginal, vulvar, and anal intraepithelial neoplasia and the respective anogenital cancers [1,2,3]. Pseudoviruses have codon optimized L1 and L2 capsid proteins but, in contrast to quasiviruses, encapsidate a GFP reporter plasmid (pseudogenome) that can be tracked microscopically in cellular infectivity assays [11,12,13,14,15]. In the past, both HPV and the polyomavirus simian virus 40 (SV40) were assigned to the family Papovaviridae, a abolished family, which was split into the two families Papillomaviridae and Polyomaviridae in 1999 [16]. We comparatively evaluated the susceptibility of HPV16 pseudovirus and SV40 to different disinfectants using quantitative suspension tests
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
