Abstract
When bacteriophage T4 is treated with the alkylating agent ethylmethane-sulfonate (EMS), inactivation of the plaque-forming capacity(primary, or immediate inactivation) follows a curve which is initially shouldered but becomes exponential at higher doses. This was interpreted by Brookes and Lawley (1963) as indicating a multi-hit mechanism of killing. However, Krieg (1963) obtained a killing curve that failed to become exponential down to 10 -6
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