Abstract

Counts of coliform bacteria in sewage sludge cakes which ranged from 1×105 to2×105 CFU per g were diminished rapidly with radiation treatments, and completely inactivated at 4 kGy of irradiation. The number of salmonellae in sewage sludge cakes and dried sludge or hospital were determined to be from 13 to 1.4×104MPN (most probable number) per 100 g, and 44 serotypes were identified. D10 values of these isolates in phosphate buffer were determined to be 0.10 to 0.23 kGy, and the necessary dose for inactivation of salmonellae in sewage sludge cakes was estimated to be 4 kGy. As antagonistic bacteria, Bacillus subtilis, B. polymyxa and other Bacillus species isolated from soils or compost seeding were effective in suppressing the growth of phytopathogenic fungi. Among these bacteria, B. subtilis had the strongest suppressive effect on phytopathogenic fungi. The growth of B. subtilis occurred rapidly during 24h cultivation in radiation-disinfected sludge. When the cultivation processwas observed in terms of CO2, evolution, the peak value was obtained after 23h incubation and fermentation was completed in a short period.

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