Inactivation of Opportunistic Pathogens Acinetobacter baumannii and Stenotrophomonas maltophilia by Antimicrobial Photodynamic Therapy.

Irina Buchovec,Edita Sužiedėlienė,Laurita Klimkaitė,Saulius Bagdonas

doi:10.3390/microorganisms10030506

Irina Buchovec, Edita Sužiedėlienė + Show 2 more

Open Access

https://doi.org/10.3390/microorganisms10030506

Copy DOI

Journal: Microorganisms	Publication Date: Feb 25, 2022
Citations: 10	License type: CC BY 4.0

Affiliation: Vilnius University

Abstract

Acinetobacter baumannii and Stenotrophomonas maltophilia are opportunistic pathogens causing hospital infections with limited treatment options due to bacterial multidrug resistance. Here, we report that antimicrobial photodynamic therapy (aPDT) based on the natural photosensitizers riboflavin and chlorophyllin inactivates A. baumannii and S. maltophilia. The riboflavin and chlorophyllin photostability experiments assessed the photomodifications of photosensitizers under the conditions subsequently used to inactivate A. baumannii and S. maltophilia. A. baumannii planktonic cells were more sensitive to riboflavin-aPDT, while biofilm bacteria were more efficiently inactivated by chlorophyllin-aPDT. S. maltophilia planktonic and biofilm cells were more susceptible to chlorophyllin-aPDT compared to riboflavin-aPDT. The results suggest that riboflavin- and chlorophyllin-aPDT can be considered as a potential antimicrobial treatment for A. baumannii and S. maltophilia inactivation.

Highlights

Infections caused by antibiotic-resistant bacteria are a global problem urgently requiring new antibacterial agents or alternative antibacterial strategies [1]
In this study we aim to investigate whether natural PS riboflavin- and chlorophyllinbased antimicrobial photodynamic therapy (aPDT) can efficiently inactivate antibiotic-resistant A. baumannii and S. maltophilia bacteria and their biofilms
The is one of the most pressing worldwide health issues. These infection agents have is one of the most pressing worldwide health issues. These infection agents have developed the ability to survive in the hospital environment and gained virulent features required for efficient infection of the host [18,38]

Summary

Introduction

Infections caused by antibiotic-resistant bacteria are a global problem urgently requiring new antibacterial agents or alternative antibacterial strategies [1]. Gram-negative opportunistic pathogens resistant to most clinically used antibiotics [2,3]. Opportunistic infections are hazardous to patients with compromised immunity, and are characterized by high morbidity and mortality [4]. The bacterium Acinetobacter baumannii is among the top infection agents for which new antimicrobial therapies are needed [5]. The prevalence of carbapenem-resistant A. baumannii in Europe reached 80% [7,8], creating the challenge of addressing these pathogen infections. Another newly emerging Gram-negative opportunistic pathogen causing serious concern is Stenotrophomonas maltophilia [9,10]. This bacterium is commonly found in nature, it becomes dangerous to patients with deficient immunity [11,12].

Objectives

Methods

Results

Conclusion