Abstract
The objective of this study was to investigate the effect of H2O2/O3inactivatingGiardia intestinali(G.intestinali) by the fluorescence staining method and vitro excystation. Results suggested that hydroxyl radicals (•OH) was the principal factor during the H2O2/O3process by testing the effect oft-butyl alcohol (TBA) and concentration change of H2O2. The inactivation rate achieved 99.6% when independent addition of O3under 1.5 mg·L-1with contact time about 10.0 min. While to achieve the same inactivation effect by H2O2/O3,1.0 mg·L-1O3([H2O2]/[O3] molar ratio = 0.8) with 7.0 min was required. The inactivating capability was stronger under acidic condition than in alkaline environment. The effect of turbidity was also found to be significant in synthetic water, when turbidity increasing, the inactivating effect declined. Inactivation rate was improved with a temperature increase from 5 to 35 °C. When dissolved organic matter concentration in the reaction system increased, the competitive reaction between G. intestinal and organics with H2O2/O3probably took place, thereby reducing the inactivation rate.
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