Abstract
Sucrose is naturally synthesized by many cyanobacteria under high salt conditions, which can be applied to produce this widely used feedstock. To improve sucrose production with the moderate halo-tolerant cyanobacterium Synechocystis sp. PCC 6803, we identified and biochemically characterized the sucrose-degrading invertase. Inactivating the invertase encoding gene sll0626 (inv) significantly increased cellular sucrose levels; interestingly sucrose over-accumulation was also observed under NaCl-free conditions. The subsequent inactivation of inv in the mutant ΔggpS, which cannot synthesize the major compatible solute glucosylglycerol, resulted in further enhanced sucrose accumulation in the presence of 1.5 % NaCl. Then, inv mutation was introduced into the previously obtained sucrose-producing strain WD25 (Du W, Liang F, Duan Y, Tan X, Lu X. Metab Eng 2013;19:17-25), which resulted in almost 40 % higher sucrose accumulation. These findings show that invertase is an interesting target in obtaining efficient sucrose production in cyanobacterial host cells.
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