Abstract
The effect of medium pressure ultraviolet radiation on Giardia muris was studied using a collimated beam apparatus with filtered surface water from the Grand River, Kitchener, Ontario, Canada. UV doses ranged from 5 to 83 mJ/cm 2 and resulted in 2–3 log-units of reduction in infectivity measured by a C3H/HeN mouse infectivity model. In vitro excystation and nucleic acid staining with Live/Dead BacLight ™ greatly underestimated the inactivation of Giardia when compared with animal infectivity. Medium pressure ultraviolet radiation is a potential alternative to conventional chemical disinfection methods.
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