Abstract
BackgroundThe activity of airborne disinfectants on bacteria, fungi and spores has been reported. However, the issue of the virucidal effect of disinfectants spread by fogging has not been studied thoroughly.MethodsA procedure has been developed to determine the virucidal activity of peracetic acid-based airborne disinfectants on a resistant non-enveloped virus poliovirus type 1. This virus was laid on a stainless carrier. The products were spread into the room by hot fogging at 55°C for 30 minutes at a concentration of 7.5 mL.m-3. Poliovirus inoculum, supplemented with 5%, heat inactivated non fat dry organic milk, were applied into the middle of the stainless steel disc and were dried under the air flow of a class II biological safety cabinet at room temperature. The Viral preparations were recovered by using flocked swabs and were titered on Vero cells using the classical Spearman-Kärber CPE reading method, the results were expressed as TCID50.ml-1.ResultsThe infectious titer of dried poliovirus inocula was kept at 105 TCID50.mL-1 up to 150 minutes at room temperature. Dried inocula exposed to airborne peracetic acid containing disinfectants were recovered at 60 and 120 minutes post-exposition and suspended in culture medium again. The cytotoxicity of disinfectant containing medium was eliminated through gel filtration columns. A 4 log reduction of infectious titer of dried poliovirus inocula exposed to peracetic-based airborne disinfectant was obtained.ConclusionThis study demonstrates that the virucidal activity of airborne disinfectants can be tested on dried poliovirus.
Highlights
The activity of airborne disinfectants on bacteria, fungi and spores has been reported
Based on procedures aimed to determine the bactericidal, fungicidal and sporicidal activities of products spread by aerial way for the disinfection of surfaces, we investigated the effect of peracetic acid-based airborne disinfectants toward poliovirus type 1, a model of nonenveloped resistant virus
Poliovirus dried on stainless carriers 50 μL of poliovirus inoculum applied in the middle of each stainless steel disc were dried under the airflow of a class II biological safety cabinet at room temperature (20 ±2°C) for 2 h (±15 min)
Summary
The activity of airborne disinfectants on bacteria, fungi and spores has been reported. The issue of the virucidal effect of disinfectants spread by fogging has not been studied thoroughly. It has been put forward that a thorough disinfection of rooms (air and surface), in a hospital environment, can be achieved thanks to hydrogen peroxide [1]. Bacterial disinfection can be obtained through fogging of product as already proved [2]. The bactericidal, fungicidal and sporicidal activities of aerial surface disinfection can be tested thanks to described procedures [3]. The transmission of viruses through contaminated surfaces has been described [4]. Non-enveloped viruses are in general more resistant to disinfection and drying than enveloped viruses. The infectivity of viruses can be maintained a few hours in
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