Inactivation, mutation induction and repair in Bacillus subtilis spores irradiated with heavy ions

Abstract

Studies on the response of bacterial spores to accelerated heavy ions (HZE particles) help in understanding problems of space radiobiology and exobiology. Layers of spores of Bacillus subtilis strains, differing in repair capabilities, were irradiated with accelerated boron, carbon and neon ions of linear energy transfer (LET) values up to 14000 MeV cm 2/g. Inactivation as measured by loss of colony forming ability and induction of mutations as measured by reversion to histidine prototrophy and resistance to 150 μg/ml sodium azide were tested, as well as the influence of repair processes on these effects. For inactivation, the cross-sectional values σ plotted as a function of LET follow a saturation curve. The plateau, which is reached around a LET of 2000 MeV cm 2/g, occurs at 2.5 × 10 −9 cm 2, a value in good agreement with the dimensions of the spore protoplast. Lethal damage produced at LET values < 2000 MeV cm 2/g is reparable. Recombination repair is more effective than excision repair. At higher LET values, lethal damage could not be reconstituted by the repair mechanisms studied. In addition, at these high LET values, the frequency of induced mutations was drastically decreased. The data support the assumption of at least two qualitatively different types of lesion, depending on the LET of the affecting heavy ion.