Inactivation efficiency of Bacillus endospores via modified flow-through PUV treatment with comparison to conventional LPUV treatment

Abstract

Water is a fragile resource, consequently there is a pressing need to develop sustainable environmental-friendly disinfection technologies. This constitutes the first study to compare efficacy of continuous low-pressure UV (LPUV) to that of pulsed UV light (PUV) for UV disinfection performance in flow-through water systems. Bacillus endospores were used as these are frequently deployed as biodosimeters for drinking water UV reactor validation protocols. Results showed a PUV system output of 2,052 mJ/cm2 (energy below 300 nm) was required for a 2 log inactivation of B.pumilus endospores, whereas a lower LPUV system output of 12 mJ/cm2 produced a similar level of disinfection. Measurements of dose incident to the samples (which were 10.75 cm from the PUV light source) showed supplementation of cultivation media with manganese sulphate monohydrate (MnSO4.H2O) employed to enhance the rate of endospore formation for propagation was shown to increase the UV resistance of different Bacillus spp. to both irradiation approaches (P < 0.05). Conventional LPUV light that is commonly-used by the drinking water industry is a reliable and superior approach to PUV which requires substantial modification for effective disinfection of continuous flow-through water. Caution must also be exercised when manipulating media formulation to propagate endospores as this may lead to inconsistencies in reporting efficacy of intervention treatments with implications for validation and environmental evaluation.