Inactivation by helium cold atmospheric pressure plasma for Escherichia coli and Staphylococcus aureus

Khaled Lotfy,Sayed Mohammed Khalil,Hany Abd El-Raheem

doi:10.1007/s40094-019-00362-4

Khaled Lotfy, Sayed Mohammed Khalil + Show 1 more

Open Access

https://doi.org/10.1007/s40094-019-00362-4

Copy DOI

Abstract

A helium cold atmospheric pressure plasma jet (HCAPPJ) driven by a commercial neon power supply was designed and utilized for inactivation bacteria. The generated reactive spices by HCAPPJ were investigated by optical emission spectroscopy. The reactive species of OH, OI, OI, N21+, N21+ and He were identified in the UV–Vis wavelength region. The reactive species was not detected between 200 nm and 300 nm, as the flow rate of helium gas increased that led to the plasma temperature reducing to a value near to the room temperature. In this work, we studied the impact of HCAPPJ on Gram-positive and Gram-negative bacteria. The survival amounts of the two types of bacteria were decreased vastly when the rate flow rate was equal to 10 L/min.

Highlights

The expression ‘plasma’ indicates a quasi-neutral ionized gas, composed initially of free electrons, ions and ground- or excited-state atoms
To study the effects of helium cold atmospheric pressure plasma jet (HCAPPJ) on bacteria inactivation, initially, the plasma temperature was measured to make sure that the used cold atmospheric pressure plasma jet is safe and has powerful impacts
The plasma temperature and the concentration reactive species depend on the applied voltage, the distance from the nozzle and the helium flow rate

Summary

Introduction

The expression ‘plasma’ indicates a quasi-neutral ionized gas, composed initially of free electrons, ions and ground- or excited-state atoms. The comprehensive components of plasma exist in a net neutral charge [1]. Plasma is the fourth state of matter and forms more than 99% of the universe. Plasma consists of active particles (e.g., electrons, ions and photons), reactive species (e.g., free radicals) and temporary fields (e.g., heat, acoustic wave, electromagnetic fields). Plasma is a very complicated and active medium. In plasma applications like treating biological materials, plasma components work harmonically

Objectives

Methods

Findings

Conclusion